Use of Real-Time Quantitative PCR to Compare DNA Isolation Methods
نویسندگان
چکیده
منابع مشابه
Quantitative Analysis of CMV-DNA Load in Renal Transplant Recipients Using Real-Time PCR
No abstract
متن کاملQuantitative Real-Time PCR
Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...
متن کاملReal time quantitative PCR.
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over conta...
متن کاملReal-time quantitative PCR.
Those who have worked in the field of quantitative polymerase chain reaction (PCR) since the early 1990s have accepted many of the tedious aspects of the early assays as routine. Difficulties associated with early quantitative PCR techniques included: (i) ensuring that the PCR was within the linear range of amplification (that portion where the PCR signal is directly proportional to the input c...
متن کاملQuantitative detection of Tropheryma whipplei DNA by real-time PCR.
Over the past decade, PCR-based methodologies have been introduced to complement or even replace histopathologic study of biopsy specimens for the diagnosis of Whipple’s disease (12). However, positive PCR results have been reported on testing small-bowel and saliva specimens from asymptomatic patients (3, 4, 11). Although these results have not been independently confirmed (8), they have nonet...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1998
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/44.10.2201